!! This is the archived website of Professor Andrew J. Millar's research group at the University of Edinburgh (to 2017) !! Current work is linked from here: http://www.amillar.org |
Luciferase The glow is widely used as an assay for LUC expression, which acts as a "reporter" for the activity of any genetic regulation that controls the expression of its gene. Luciferase is particularly useful as a reporter in living cells and organisms. LUC gene fusions provide a "window" on to the mechanisms that regulate the activity of specific genes, in specific, living cells. Our interest is in the mechanism of rhythmic regulation in higher plants: a rhythmically-expressed luciferase reporter has been one of our principal tools since 1990 (published in Millar et al. Plant Mol Biol Rep 1992 (Arabidopsis); Millar et al. Plant Cell 1992 (tobacco)). Images of bioluminescence from our CAB:LUC Arabidopsis plants are shown at the top of this page (the cotyledons of two seedlings) and on the front page (an older, rosette plant). Several different luciferase genes are now available from commercial suppliers. We most commonly use the LUC+ gene from Promega Corporation. The protein is cytoplasmic, whereas the native LUC is peroxisomal. Luciferase Links
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