Leaf movement imaging
The original, low-cost system for timelapse analysis of plant growth uses 14 security
video cameras controlled by a single PC, known as the "Kujata". The Kujata
system was designed for the Kay lab at the University of Virgina, in 1994, and is available
custom-built from Mark Hyett (email@example.com).
It is efficient but no longer current technology, in part because it is based on the VESA
video standard. The leaf movement and hypocotyl videos were taken
with this system.
In 1999-2000, Andrew Millar and Kieron Edwards developed a new version known as
KujaMorph, which uses almost entirely standard components under the control of MetaMorph
Video technology is moving quickly and to much lower
costs, so there should (by 2003) be a better solution for someone who wants to
put one together, perhaps based on USB web cameras ?
|Lenses: 8mm fixed-focus, manual-iris|
|Cameras: inexpensive B/W security cameras, 380 TV lines|
|Camera holders: adjustable, three-leg platform designed by Paul Goode and the Biol. Sci.
|Video switchers: Two Videoswitch VSX416, for 31 cameras. Any switcher with an alarm port
will do, otherwise software drivers are required to change the channel.
Many video switches have serial ports, which MetaMorph can also control with
|Control: custom parallel port cable to Videoswitch alarm port allows MetaMorph to change
video channel without software drivers|
|Framegrabber: Flashbus Pro. Any framegrabber supported by Metamorph will do.|
|Software: MetaMorph with Custom I/O control, Motion detection and other options.|
|Housed in temperature-controlled plant growth room in constant fluorescent light, with
video cables to three Sanyo Gallenkamp ML350 illuminated growth chambers.|
|Sample holders: 25-cell tissue culture dishes for Arabidopsis seedlings, 2-inch
strip pots on wooden mounts for Brassica.|
Notes and gripes
|Cameras should be gen-locked - we have sporadic
flagging on the images and other synch problems, which sometimes lead
the system to freeze if MetaMorph can't grab a frame. An error message over-ride in
MetaMorph would solve the problem, all suggestions gratefully received.|
|Removing the switcher's text overlay would be useful|
|MetaMorph drivers for Flashbus Pro framegrabber
board support fewer acquisition
channels than the board.|
(see also Movies for labs with alternative cameras for imaging